Journal
JOURNAL OF BIOCHEMISTRY
Volume 159, Issue 6, Pages 585-597Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvw002
Keywords
2-arachidonoylglycerol; diacylglycerol; endocannabinoid; lipase; phospholipase
Categories
Funding
- JSPS KAKENHI Grant [23592275, 24390069, 23659106]
- Grants-in-Aid for Scientific Research [23592275, 23659106, 25440080, 24390069] Funding Source: KAKEN
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Diacylglycerol (DG) lipase, which hydrolyses 1-stearoyl-2-arachidonyl-sn-glycerol to produce an endocannabinoid, 2-arachidonoylglycerol, was purified from the soluble fraction of rat brain lysates. DG lipase was purified about 1,200-fold by a sequential column chromatographic procedure. Among proteins identified by mass spectrometry analysis in the partially purified DG lipase sample, only DDHD domain containing two (DDHD2), which was formerly regarded as a phospholipase A(1), exhibited significant DG lipase activity. Rat DDHD2 expressed in Chinese hamster ovary cells showed similar enzymatic properties to partially purified DG lipase from rat brain. The source of DG lipase activity in rat brain was immunoprecipitated using anti-DDHD2 antibody. Thus, we concluded that the DG lipase activity in the soluble fraction of rat brain is derived from DDHD2. DDHD2 is distributed widely in the rat brain. Immunohistochemical analysis revealed that DDHD2 is expressed in hippocampal neurons, but not in glia.
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