Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 32, Issue 2, Pages 209-222Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911516671150
Keywords
Chitosan; thermosensitive chitosan gels; structural properties; cell culture; biomaterial; biocompatibility; scaffolds; tissue engineering; calcium glycerophosphate
Funding
- National Science Centre of Poland [NCN UMO-2011/01/B/ST8/06686, NCN UMO-2014/15/B/ST8/02512]
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In this article, properties of thermosensitive chitosan hydrogels prepared with the use of chitosan chloride with -glycerophosphate disodium salt pentahydrate enriched with calcium glycerophosphate are presented and compared with chitosan hydrogels with -glycerophosphate disodium salt pentahydrate. The study is focused on the determination of hydrogel structure and biological testing of hydrogels with human osteoblasts line Saos-2. The structure of gels was visualized by scanning electron microscopy and was investigated by infrared spectroscopy. The crystallinity of gel structure was determined by X-ray diffraction analysis and thermal effects were determined using differential scanning calorimetry thermograms.
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