Journal
CELL REPORTS
Volume 38, Issue 10, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2022.110494
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Funding
- National Institutes of Health [NIAID R01Al137078]
- Department of Defense [W81XWH-17-PRMRP]
- Johns Hopkins Malaria Research Institute postdoctoral fellowship
- Johns Hopkins Malaria Research Institute
- Bloomberg Philanthropies
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In mosquitoes, ectopic expression of AgOR inhibits the activity of expressing neurons and leads to significant downregulation of most AgOR transcripts. Additionally, the gene choice of AgOR remains active into adulthood and the expression of AgOR2 inhibits the turning on of AgORs at this late stage.
Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expressing neuron. This contrasts with studies in Drosophila melanogaster in which the ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. RNA-seq analyses comparing wild-type antennae to those ectopically expressing AgOR2 in ORNs indicated that nearly all AgOR transcripts were significantly downregulated (except for AgOR2). Additional experiments suggest that AgOR2 protein rather than mRNA mediates this downregulation. Using in situ hybridization, we find that AgOR gene choice is active into adulthood and that AgOR2 expression inhibits AgORs from turning on at this late stage. Our study shows that the ORNs of Anopheles mosquitoes (in contrast to Drosophila) are sensitive to a currently unexplored mechanism of AgOR regulation.
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