Gut microbial dysbiosis in rheumatoid arthritis: a systematic review protocol of case-control studies

Introduction Rheumatoid arthritis (RA) has a huge societal impact due to the high prevalence, irreversible joint damage and systemic complications. Gut microbiota plays an important role in the pathogenesis and progression of RA by regulating the host immune system. Restoring intestinal homeostasis by altering the microbiota could be an attractive strategy for the prevention and treatment of RA. However, the signature features of microbial dysbiosis in RA are still controversial. Therefore, we aim to elucidate the characteristic change in the diversity and composition of gut microbiota in RA. Methods and analysis We will systematically search through PubMed, EMBASE, Web of Science and Cochrane Library, as well as dissertations and conference proceedings. The reference lists of all included studies will be also reviewed to retrieve additional relevant studies. The case-control studies that reported either the relative abundance of bacteria at the phylum or genus level or at least one of the alpha-diversity, beta-diversity indexes in both RA and healthy controls will be included. Eligible studies will be screened independently by two reviewers according to the inclusion criteria. The Newcastle-Ottawa Quality Assessment Scale will be used to assess the quality of the included studies. Data extraction, qualitative and quantitative analysis will be performed within the gut microbial dysbiosis in RA. The expected outcomes will be the identification of the specific changes in composition and diversity of the gut microbiota in patients with RA. The quality of evidence will be assessed by the Grading of Recommendations Assessment, Development and Evaluation framework. Ethics and dissemination Ethical approval is unnecessary as this review does not address the data and privacy of patients. The results will be published in a peer-reviewed scientific journal and conference presentations. PROSPERO registration number CRD42021225229.

Automated summary

This study aims to elucidate the characteristic change in the diversity and composition of gut microbiota in patients with rheumatoid arthritis (RA), in order to understand its role in the pathogenesis and progression of RA. A systematic search of multiple databases will be conducted, and case-control studies meeting the inclusion criteria will be independently screened. The quality of evidence will be assessed using the Grading of Recommendations Assessment, Development and Evaluation framework. The results will be published in a peer-reviewed scientific journal and presented at conferences.