4.7 Article

Intimate relationships among actinomycetes and mycolic acid-containing bacteria

Journal

SCIENTIFIC REPORTS
Volume 12, Issue 1, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41598-022-11406-2

Keywords

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Funding

  1. IFO, Institute for Fermentation, Osaka
  2. Amano Enzyme Foundation
  3. JSPS A3 Foresight Program
  4. JSPS KAKENHI [16K18673, 18H02120]
  5. Grants-in-Aid for Scientific Research [18H02120, 16K18673] Funding Source: KAKEN

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This study explores the interaction between a naturally co-isolated pair of actinomycetes and MACB. The experiment demonstrates that the actinomycetes and MACB can co-aggregate in liquid culture and exhibit metabolic changes. Additionally, actinomycetes and MACB with co-aggregation characteristics were also found in soil.
Co-culture is an efficient strategy for natural product discovery. We have used mycolic acid-containing bacteria (MACB) Tsukamurella pumonis TP-B0596 to induce secondary metabolism by actinomycetes and have found several natural products. We also observed that MACB attached to the mycelium of Streptomyces lividans forming coaggregates during combined-culture. This stimulated interest in the interactions among actinomycetes and MACB, and we found that soil isolated cultures contained a mixture of actinomycetes and MACB. Our previously observed interactions were the result of selective screening and combination of bacteria in the lab, which warranted investigation of the existence of these interactions in the natural soil environment. Therefore, in this paper, we report the interaction between a co-isolated natural pair of actinomycetes and MACB in terms of morphology and metabolic changes. A natural pair of actinomycetes and MACB co-aggregated in liquid culture and showed metabolic changes. Interestingly, co-aggregated actinomycetes and MACB were re-isolated from soil with no obvious morphological colony differences from the colony of a single strain. The results demonstrate that there is a stochastic chance of picking colonies containing co-aggregated actinomycetes and MACB, which suggests that the pair can exist in co-aggregate form in the soil environment and interact with each other.

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