Characterizations of botanical attractant of Halyomorpha halys and selection of relevant deorphanization candidates via computational approach

Halyomorpha halys has been recognized as a global cross-border pest species. Along with well-established pheromone trapping approaches, there have been many attempts to utilize botanical odorant baits for field monitoring. Due to sensitivity, ecological friendliness, and cost-effectiveness for large-scale implementation, the selection of botanical volatiles as luring ingredients and/or synergists for H. halys is needed. In the current work, botanical volatiles were tested by olfactometer and electrophysiological tests. Results showed that linalool oxide was a potential candidate for application as a behavioral modifying chemical. It drove remarkable attractiveness toward H. halys adults in Y-tube assays, as well as eliciting robust electroantennographic responsiveness towards antennae. A computational pipeline was carried out to screen olfactory proteins related to the reception of linalool oxide. Simulated docking activities of four H. halys odorant receptors and two odorant binding proteins to linalool oxide and nerolidol were performed. Results showed that all tested olfactory genes were likely to be involved in plant volatile-sensing pathways, and they tuned broadly to tested components. The current work provides insights into the later development of field demonstration strategies using linalool oxide and its molecular targets.

Automated summary

This study investigates the potential use of botanical volatiles, specifically linalool oxide, as a behavioral modifying chemical for Halyomorpha halys. The results show that linalool oxide exhibits remarkable attractiveness towards H. halys adults and elicits strong electroantennographic responsiveness. Computational screening of olfactory proteins related to linalool oxide reception suggests their involvement in plant volatile-sensing pathways. This study provides valuable insights for the development of field demonstration strategies.