Journal
JOURNAL OF COSMETIC DERMATOLOGY
Volume 21, Issue 10, Pages 5026-5036Publisher
WILEY
DOI: 10.1111/jocd.14956
Keywords
amniotic fluid derived stem cell; CXCR4; exosomal microRNAs; wound healing
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Funding
- Suzhou Science and Technology Development Plan [SYS2020109]
- Natural Science Foundation of Jiangsu Province [BK20160349]
- Colleges and Universities Natural Science Research Project of Jiangsu Province [19KJB320006]
- Project of State Key Laboratory of Radiation Medicine and Protection, Soochow University [GZK1202107]
- National Natural Science Foundation of China [31600790, 31771046]
- Jiangsu Provincial Medical Youth Talent [QNRC2016770]
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This study investigated the effects of exosomes derived from human amniotic fluid derived stem cells (hAFS) in wound healing. The results showed that hAFS accelerated wound closure and alleviated scar formation by promoting ECM remodeling, upregulating molecular of immune response, enhancing anti-fibrotic activity, and decreasing the secretion of inflammation-associated cytokines through exosomal miRNA-146a-5p via targeting CXCR4. These findings suggest that hAFS could be a promising cell source for wound healing.
Background and Objectives Regenerative medicine is promising in wound healing. Exosomes derived from human amniotic fluid derived stem cells (hAFS) have become an important area of research for many diseases as a key paracrine factor, but its effects in wound healing remains unknown. In this study, we investigated the possible role and possible mechanisms of hAFS in skin wound healing. Methods hAFS were isolated from human amniotic fluid via routine amniocentesis. The mice were randomly divided into 2 groups: control group and hAFS group treated with 1.25 x 10(6) hAFS cells. Immunohistochemistry staining was performed for histological analysis and qRT-PCR for the assessment of gene levels. Luciferase Reporter Assay was performed for the verification of target gene. Results Our results demonstrated that hAFS accelerated wound closure. hAFS alleviated scar formation via promoting ECM remodeling, upregulating molecular of immune response, enhancing anti-fibrotic activity, and decreasing the secretion of inflammation-associated cytokines through exosomal miRNA-146a-5p via targeting CXCR4. Conclusions Taken together, hAFS was a promising cell source for wound healing. The findings in this study provide vital references and pave the way for future research.
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