First report of flavonoids from leaves of Machaerium acutifolium by DI-ESI-MS/MS

The aqueous fraction obtained by the partition of the ethanolic extract from leaves of Machaerium acutifolium (Fabaceae-Papilionoideae) was analyzed by high-performance liquid chromatography with a diode array detector (HPLC-DAD) and direct insertion in a mass spectrometer with an ion trap analyzer equipped with an electrospray ionization source (DI-ESI-MS/MS). The chemical analysis of the extract demonstrated the occurrence of eight flavonols (1-8), two isoflavonoids (9 and 10) and one biflavonoid (11). These compounds are being reported for the first time from M. acutifolium. The aqueous fraction showed 28.37 +/- 0.94% of AA in assay on DPPH and 151.70 +/- 9.44 GAE of the total phenolic content. (c) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Automated summary

The aqueous fraction of Machaerium acutifolium leaves extract was analyzed and found to contain various flavonoids and phenolic compounds. The aqueous fraction exhibited significant antioxidant activity and a high total phenolic content.