4.8 Article

FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA

Russell T. Walton et al.

Summary: The protocol of HT-PAMDA allows for scalable characterization of the PAM preferences of different Cas proteins, enabling comprehensive comparison and profiling of natural and engineered enzymes in a cost-effective and efficient manner, making it a valuable tool for CRISPR-Cas research.

NATURE PROTOCOLS (2021)

Article Biochemistry & Molecular Biology

PpCas9 from Pasteurella pneumotropica - a compact Type II-C Cas9 ortholog active in human cells

Iana Fedorova et al.

NUCLEIC ACIDS RESEARCH (2020)

Article Multidisciplinary Sciences

A catalogue of biochemically diverse CRISPR-Cas9 orthologs

Giedrius Gasiunas et al.

NATURE COMMUNICATIONS (2020)

Article Biotechnology & Applied Microbiology

A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

Peng Chen et al.

GENOME BIOLOGY (2020)

Article Biotechnology & Applied Microbiology

Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

Fei Teng et al.

GENOME BIOLOGY (2019)

Article Mathematical & Computational Biology

CasPDB: an integrated and annotated database for Cas proteins from bacteria and archaea

Zhongjie Tang et al.

DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION (2019)

Article Biochemical Research Methods

fastp: an ultra-fast all-in-one FASTQ preprocessor

Shifu Chen et al.

BIOINFORMATICS (2018)

Article Biochemistry & Molecular Biology

CRISPRMatch: An Automatic Calculation and Visualization Tool for High-throughput CRISPR Genome-editing Data Analysis

Qi You et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES (2018)

Article Biochemistry & Molecular Biology

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

Hao Yin et al.

NATURE CHEMICAL BIOLOGY (2018)

Article Multidisciplinary Sciences

Minimal PAM specificity of a highly similar SpCas9 ortholog

Pranam Chatterjee et al.

SCIENCE ADVANCES (2018)

Article Multidisciplinary Sciences

New CRISPR-Cas systems from uncultivated microbes

David Burstein et al.

NATURE (2017)

Article Multidisciplinary Sciences

Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage

Nicole M. Gaudelli et al.

NATURE (2017)

Article Multidisciplinary Sciences

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

Eunji Kim et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

A thermostable Cas9 with increased lifetime in human plasma

Lucas B. Harrington et al.

NATURE COMMUNICATIONS (2017)

Article Biochemistry & Molecular Biology

ClinVar: public archive of interpretations of clinically relevant variants

Melissa J. Landrum et al.

NUCLEIC ACIDS RESEARCH (2016)

Article Multidisciplinary Sciences

Rationally engineered Cas9 nucleases with improved specificity

Ian M. Slaymaker et al.

SCIENCE (2016)

Article Multidisciplinary Sciences

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Benjamin P. Kleinstiver et al.

NATURE (2016)

Letter Biotechnology & Applied Microbiology

Open-source guideseq software for analysis of GUIDE-seq data

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2016)

Article Biotechnology & Applied Microbiology

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench et al.

NATURE BIOTECHNOLOGY (2016)

Article Biochemistry & Molecular Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche et al.

Article Multidisciplinary Sciences

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver et al.

NATURE (2015)

Article Medicine, Research & Experimental

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy

Zheng Hu et al.

JOURNAL OF CLINICAL INVESTIGATION (2015)

Article Biotechnology & Applied Microbiology

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Hiroshi Nishimasu et al.

Review Biochemistry & Molecular Biology

Development and Applications of CRISPR-Cas9 for Genome Engineering

Patrick D. Hsu et al.

Article Biochemistry & Molecular Biology

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

Seung Woo Cho et al.

GENOME RESEARCH (2014)

Article Biotechnology & Applied Microbiology

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

Yanfang Fu et al.

NATURE BIOTECHNOLOGY (2014)

Article Biochemical Research Methods

STAR: ultrafast universal RNA-seq aligner

Alexander Dobin et al.

BIOINFORMATICS (2013)

Article Biotechnology & Applied Microbiology

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Prashant Mali et al.

NATURE BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu et al.

NATURE BIOTECHNOLOGY (2013)

Article Biochemical Research Methods

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Kevin M. Esvelt et al.

NATURE METHODS (2013)

Article Multidisciplinary Sciences

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

Zhonggang Hou et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Multidisciplinary Sciences

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong et al.

SCIENCE (2013)

Article Multidisciplinary Sciences

Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System

Alfonso H. Magadan et al.

PLOS ONE (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Biochemical Research Methods

FLASH: fast length adjustment of short reads to improve genome assemblies

Tanja Magoc et al.

BIOINFORMATICS (2011)

Article Chemistry, Multidisciplinary

NUPACK: Analysis and Design of Nucleic Acid Systems

Joseph N. Zadeh et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2011)

Review Biochemistry & Molecular Biology

TATA box polymorphisms in human gene promoters and associated hereditary pathologies

L. K. Savinkova et al.

BIOCHEMISTRY-MOSCOW (2009)

Article Biochemical Research Methods

Fast and accurate short read alignment with Burrows-Wheeler transform

Heng Li et al.

BIOINFORMATICS (2009)

Article Biochemistry & Molecular Biology

FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix

Morgan N. Price et al.

MOLECULAR BIOLOGY AND EVOLUTION (2009)

Article Biochemical Research Methods

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson et al.

NATURE METHODS (2009)

Article Biochemistry & Molecular Biology

The HHpred interactive server for protein homology detection and structure prediction

J Söding et al.

NUCLEIC ACIDS RESEARCH (2005)

Article Biochemistry & Molecular Biology

MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform

K Katoh et al.

NUCLEIC ACIDS RESEARCH (2002)