Journal
NATURE COMMUNICATIONS
Volume 13, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-29368-4
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Funding
- Austrian Science Fund (FWF) [P30584, P33066, W1261]
- Michael Smith Foundation for Health Research (MSFHR) Scholar award [17686]
- Cancer Research Society [CRS-24368]
- Austrian Science Fund (FWF) [P33066, P30584, W1261] Funding Source: Austrian Science Fund (FWF)
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This study elucidates the mechanism of self-activation of 3-phosphoinositide-dependent kinase 1 (PDK1). It is activated by dimerization mediated by PIP3 and trans-autophosphorylation, while its PH domain inhibits its activity. These results suggest that phosphoinositide-dependent activation may also apply to various effector kinases.
3-phosphoinositide-dependent kinase 1 (PDK1) is an essential serine/threonine protein kinase, which plays a crucial role in cell growth and proliferation. It is often referred to as a 'master' kinase due to its ability to activate at least 23 downstream protein kinases implicated in various signaling pathways. In this study, we have elucidated the mechanism of phosphoinositide-driven PDK1 auto-activation. We show that PDK1 trans-autophosphorylation is mediated by a PIP3-mediated face-to-face dimer. We report regulatory motifs in the kinase-PH interdomain linker that allosterically activate PDK1 autophosphorylation via a linker-swapped dimer mechanism. Finally, we show that PDK1 is autoinhibited by its PH domain and that positive cooperativity of PIP3 binding drives switch-like activation of PDK1. These results imply that the PDK1-mediated activation of effector kinases, including Akt, PKC, Sgk, S6K and RSK, many of whom are not directly regulated by phosphoinositides, is also likely to be dependent on PIP3 or PI(3,4)P-2. The essential protein kinase PDK1 is activated by phospoinositide-mediated dimerization and trans-autophosphorylation. Here, the authors show that in the absence of PIP3 or PI(3,4)P-2 phosphoinositides, PDK1 is maintained in an inactive, autoinhibited conformation in the cytosol.
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