Journal
CELL DEATH & DISEASE
Volume 13, Issue 4, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41419-022-04837-2
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Funding
- National Natural Science Foundation of China [82171605, 81971444, 81901567]
- Science Technology and Innovation Commission of Shenzhen Municipality [JCYJ20170818160910316]
- Hubei Provincial Natural Science Foundation of China [2018CFB111]
- National Institute of Health [GM122776, GM141085]
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This study reveals the importance of UHRF1 in the epigenetic regulation of Sertoli cells during spermatogenesis. UHRF1 regulates the expression of extracellular matrix-related genes and establishes the crosstalk between Sertoli cells and germ cells.
Sertoli cells (SCs) support and nourish germ cells (GCs) through their crosstalk during spermatogenesis. However, the underlying epigenetic mechanism that ensures SCs' functions in this process remains unclear. Here, we report that UHRF1, a critical epigenetic regulator, is mainly expressed in human and mouse pre-mature SCs, and is essential for establishing Sertoli-Germ cell crosstalk. SC-specific UHRF1 knockout mice exhibit complete sterility with Sertoli cell (SC) proliferation and differentiation aberrance, blood-testis barrier (BTB) disruption, and immature germ cell (GC) sloughing. RNA sequencing and Whole Genome Bisulfite Sequencing (WGBS) revealed that many extracellular matrix (ECM)-related genes (e.g., Timp1, Trf, and Spp1) appeared upregulated with the DNA hypomethylation status in UHRF1-deficient SCs. Strikingly, overexpression of Timp1, Trf, and Spp1 in SCs in vitro and in vivo could phenocopy the SC-specific UHRF1-deficient mice. Our data demonstrated that UHRF1 regulates the transcriptional program of ECM-related genes in SCs and establishes SC-GC crosstalk.
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