Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 90, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jff.2022.104992
Keywords
Hatakabb; Anti-inflammation; TNF-alpha; NF-kappa B; HMOX-1; Pyrogallol
Categories
Funding
- NSTDA [P2050200]
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This study demonstrated the anti-inflammatory potential and mechanism of Hatakabb herbal extract, with pyrogallol identified as a novel mediator inhibiting LPS-induced inflammation.
Hatakabb is a herbal extract traditionally consumed to ameliorate inflammation from pharynx infections. However, the anti-inflammatory function of Hatakabb was not investigated. This study demonstrated the anti-inflammatory potential, and underlying mechanism of Hatakabb, and its functional ingredient. In LPS-treated macrophages, Hatakabb treatment attenuated TNF-alpha production. A decrease in TNF-alpha, IL-6, and COX-2 expression was detected. In regulatory pathway analysis, Hatakabb suppressed I kappa B-alpha phosphorylation in accordance with a decrease in I kappa B-alpha degradation, and nucleus NF-kappa B translocation, while enhanced NF-kappa B phosphorylation. In addition, MAPK phosphorylation was enhanced in association with HMOX-1 upregulation. Our metabolomic analysis showed gallic acid, and pyrogallol contained in the anti-inflammatory subfraction. Compared to gallic acid, pyrogallol strongly diminished TNF-alpha production. Significant changes in inflammatory gene expression, and regulatory protein phosphorylation were responded to pyrogallol in similar to Hatakabb treatment. Our study, for the first time, reveals the anti-inflammatory effect, and mechanism of Hatakabb in which pyrogallol is the novel mediator inhibiting LPS-induced inflammation.
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