Journal
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
Volume 19, Issue 4, Pages 1117-1123Publisher
KOREAN SOC APPLIED ENTOMOLOGY
DOI: 10.1016/j.aspen.2016.10.006
Keywords
Stomoxyinae; Mitochondrial DNA; Cytochrome oxidase subunit 1; lnterspecific divergence
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Funding
- Mahidol University
- ICTM grant
- Faculty of Tropical Medicine, Mahidol University, Thailand
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Stomoxyinae flies (Diptera: Muscidae) transmit many pathogens to animals and humans. However, morphological identification of Stomoxyinae flies is difficult, and there is risk of misidentification. Therefore, this study analyzed cytochrome oxidase 1 (coxl))barcodes to test their ability to discriminate between taxonomic groups. coxl barcodes (657 bp) from 66 specimens were classified into 8 species. coxl sequences have typical insect mitochondrial characteristics including a high AT content (mean = 69%), especially at the third codon position. coxl barcodes were able to discriminate between species with high interspecific divergence, especially those that are morphologically difficult to distinguish, such as certain Stomoxys species (S. indices, S. pullus, and S. uruma) and Haematobia sub-species (H. irritans irritans and H. irritans exigua). Phylogenetic analysis and the genealogical sorting index revealed that all Stomoxyinae species formed a monophyletic group and were clearly separated into distinct clusters based on species. Species delimitation by the Poisson tree processes model indicated previously unrecognized diversity within Stomoxys and Haematobosca. Thus, DNA barcoding was able to discriminate between morphologically uncertain or misidentified Stomoxyinae flies and confirm the correct species, which are important steps for elucidating diversity. (C) 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
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