Journal
VIRUSES-BASEL
Volume 14, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/v14040717
Keywords
surface plasmon resonance (SPR); biolayer interferometry (BLI); virus; virus-like particle (VLP); binding characterization; diagnostics
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This review summarizes and evaluates the applications of surface plasmon resonance and biolayer interferometry, two commonly used real-time and label-free assays, in studies on virus-ligand interactions and virus-like particle-ligand binding. By examining and assessing the use of these methods, this review guides the field towards applying these robust techniques in whole virus-based studies.
Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus-ligand and virus-like particle-ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies.
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