Journal
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
Volume 26, Issue 5, Pages 502-515Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10498850.2016.1222560
Keywords
Sea cucumber; collagen; Acaudina leucoprocta; extraction; pepsin
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Funding
- Science Technology Department of Zhejiang Province [2012C37042]
- Scientific Research Fund of Zhejiang Provincial Education Department [Y201224739]
- Zhejiang Bureau of Quality and Technical Supervision [20110307]
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Sea cucumber Acaudina leucoprocta is a potential alternative collagen source. However, the high levels of heavy metals contained in the body wall restricts its utilization. In this work, an efficient method was established to remove the heavy metals accumulated in the body wall of A.leucoprocta by demineralizing with 0.2 M ethylenediaminetetraacetic acid (EDTA). The resulting body wall of A.leucoprocta was then used for extracting pepsin-soluble collagen (PSC) with pepsin proteolysis. The PSC from the body wall of A.leucoprocta was obtained with a yield of 43.99 +/- 0.65% (dry weight) and high purity. Maximum and minimum solubility for the isolated PSC in 0.5 M acetic acid was observed at pH 2.66 and 4.43, respectively. The solubility was remarkably decreased in the presence of NaCl. The denaturation temperature of PSC rehydrated in 0.5 M acetic acid was measured as 25.4 degrees C. The PSC was characterized as type I collagen, which consists of three alpha(1) chains without alpha(2) chain. Interestingly, alpha(1) chain in PSC showed two isoforms with the pI values of 4.02 and 4.29. The heavy metals existing in PSC were all below the contaminant limit of edible gelatin. The PSC isolated from the body wall could be an alternative to mammalian collagens.
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