Porcine circovirus 4 rescued from an infectious clone is replicable and pathogenic in vivo

Article Genetics & Heredity

Simultaneous detection and genetic characterization of porcine circovirus 2 and 4 in Henan province of China

Tong Xu et al.

Summary: PCV4 was identified as a novel porcine circovirus in China in 2019. A study in Henan province investigated the prevalence and genetic characteristics of PCV2 and PCV4 in 133 clinical samples, revealing higher PCV2 positive rate than PCV4 and co-infection occurrences. Sequence analysis of PCV2 strains suggested the circulation of new variant strains in Henan province, while putative genotypes of PCV4 strains were determined based on genomic sequences.

GENE (2022)

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Article Biochemical Research Methods

Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay

Da-Young Kim et al.

Summary: A novel LAMP assay for the visual detection of PCV4 has been developed with high sensitivity and specificity, resulting in higher detection rates in clinical samples compared to conventional PCR methods. This low-cost and easy-to-use assay can be a valuable tool for detecting PCV4, even in resource-limited laboratories.

JOURNAL OF VIROLOGICAL METHODS (2022)

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Article Infectious Diseases

Molecular-based detection, genetic characterization and phylogenetic analysis of porcine circovirus 4 from Korean domestic swine farms

Van-Giap Nguyen et al.

Summary: The novel and unclassified porcine circovirus 4 (PCV4) was first reported in China in 2019 and has been found to be actively circulating in pigs in both China and Korea. The Korean PCV4 strain shares high genetic similarity with other known PCV4 strains. The study also identified key genomic features and predicted essential replication elements for circoviruses in PCV4 strains, contributing to a better understanding of the genetic relationships between PCV4 and other CRESS DNA viruses.

TRANSBOUNDARY AND EMERGING DISEASES (2022)

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Letter Multidisciplinary Sciences

Viruses from poultry and livestock pose continuous threats to human beings

Si Chen et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

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Article Biochemical Research Methods

Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green I-based duplex quantitative PCR assay

Cheng-Yao Hou et al.

Summary: A novel duplex qPCR assay was developed to detect and differentiate PCV3 and PCV4 simultaneously, showing higher detection rate than conventional PCR assay and providing technical support for clinical detection, differential diagnosis and control of the viruses.

JOURNAL OF VIROLOGICAL METHODS (2021)

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Article Infectious Diseases

The seroprevalence of the newly identified porcine circovirus type 4 in China investigated by an enzymed-linked immunosorbent assay

Meng Ge et al.

Summary: PCV4 is the fourth porcine circovirus identified in China and can be detected in both diseased and healthy pigs. An indirect anti-PCV4 IgG ELISA based on Rep protein was developed and used to investigate the seroprevalence of PCV4 in pig herds in China, revealing an overall seroprevalence of 43.97%, with the highest rates detected in sows.

TRANSBOUNDARY AND EMERGING DISEASES (2021)

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Article Infectious Diseases