Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 151, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2022.116582
Keywords
Catalytic hairpin assembly; Fluorescent biosensor; Circuit leakage; Signal generation; Signal transduction; Nucleic acid circuit; DNAzyme; Nano; micromaterial
Categories
Funding
- National Natural Science Foundation of China [21874095, 21804107]
- Natural Science Foundation of Shaanxi Province, China [2020JQ-573]
- Key Research and Development Program in Shaanxi Province of China [2019ZDLSF01-03]
- Project of Sichuan Provincial Science and Technology Department [2018HH0147]
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Catalytic hairpin assembly (CHA) is a promising molecular amplifier with advantages of high efficiency, flexible design, and mild reaction condition. In recent years, CHA has been coupled with multiple signal generation and signal transduction techniques to develop diversified fluorescent biosensors. The integration of emerging signal amplification techniques and other advanced technologies has further enhanced the application of CHA-based biosensors.
Catalytic hairpin assembly (CHA) with advantages of high efficiency, flexible design, and mild reaction condition is considered a promising molecular amplifier. Modified working principle of CHAs has been coupled with multiples signal generation and signal transductions to develop the diversified fluorescent biosensors. Emerging signal amplification techniques were introduced into CHA to meet the requirement of rapid, sensitive, accurate, and specific point-of-care testing (POCT) in vitro. Moreover, others advanced techniques were integrated with CHA to improve the delivery efficiency, enhance the ability, and accelerate reaction speed for in vivo analysis. Thus, the CHA-based fluorescent biosensor has been applied on varying targets, including nucleic acids, metal ions, small molecules, proteins, enzymes, exosomes, and cells. Herein, the review systematically summarized the design principle, evolution, and application of the biosensors, and then discussed the challenges in depth. The aim was to highlight the importance of developing advanced CHA-based biosensors and promoting their practical application.(c) 2022 Elsevier B.V. All rights reserved.
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