Journal
TOXICOLOGY LETTERS
Volume 356, Issue -, Pages 151-160Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2021.12.012
Keywords
Zebrafish; MeHg; Hair cell; Hydrogen peroxide; Gene expression; Oxidative stress
Categories
Funding
- National Natural Science Foundation of China [21577136, 42177259]
- Helmholtz Association's BioInterfaces Program
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Hearing impairment and deafness can occur in patients with Minamata disease caused by methylmercury poisoning. Using zebrafish embryos, researchers found that MeHg exposure led to a concentration-dependent reduction of hair cells, in which leukocytes were involved. The loss of hair cells can be attenuated by co-treating MeHg-exposed embryos with pharmacological inhibitors of NADPH oxidases. Gene expression analysis also revealed the involvement of genes related to combating oxidative stress and immune responses, as well as hair cell regeneration, in the adverse effects of MeHg exposure.
Hearing impairment and deafness is frequently observed as one of the neurological signs in patients with Minamata disease caused by methylmercury (MeHg) poisoning. Loss of hair cells in humans and animals is a consequence of MeHg poisoning. However, it is still not clear how MeHg causes hearing deficits. We employed the hair cells of the lateral line system of zebrafish embryos as a model to explore this question. We exposed transgenic zebrafish embryos to MeHg (30-360 mg/L) at the different stages, and scored the numbers of hair cells. We find that MeHg-induced reduction of hair cells is in a concentration dependent manner. By employing antisense morpholino against to pu.1, we confirm that loss of hair cells involves the action of leukocytes. Moreover, hair cell loss is attenuated by co-treating MeHg-exposed embryos with pharmacological inhibitors of NADPH oxidases named diphenyleneiodonium (DPI) and VAS2870. In situ gene expression analysis showed that genes encoding the SQSTM1-Keap1-Nrf2 systems involved in combating oxidative stress and immune responses are highly expressed in the lateral line organs of embryos exposed to MeHg. This suggests that induction of hydrogen peroxide (H2O2) is the primary effect of MeHg on the hair cells. Genes induced by MeHg are also involved in regeneration of the hair cells. These features are likely related to the capacity of the zebrafish to regenerate the lost hair cells. (c) 2021 Elsevier B.V. All rights reserved.
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