Journal
TETRAHEDRON LETTERS
Volume 92, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2022.153678
Keywords
Labeling; Deoxynucleic acids; Uridine variant; Click chemistry; 5AmU
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Funding
- Huaz-hong University of Science and Technology
- One-Hundred-Talents Youth Program of Hubei Province
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We report the efficient synthesis and application of 2'-azidomethyl modified uridine nucleoside (2'-AmU) in labeling the nascent deoxynucleic acid of interest. This new DNA labeling tool using uridine variant (2'-AmU) has significant application potential due to its advantages, including availability, minimal size perturbation, reversible regulation, and easy click reaction.
We report here the 2'-azidomethyl modified uridine nucleoside (2'-AmU) can be efficiently synthesized and employed in the labeling study of nascent deoxynucleic acid of interest using fluorophore with alkyne (DBCO-Cy5). This new DNA labeling tool using uridine variant (2'-AmU) displays intensive application potential considering the several advantages it possesses, including readily available, rather small size (minimal perturbation), reversible regulation and easy subjected to click reaction. (C) 2022 Elsevier Ltd. All rights reserved.
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