Incorporation of azido functionality into prenylated molecules enable the fluorescent labeling of prenylated RNA in living cells

We report here the use of azidotrimethylsilane/ Selectfluor via single electron transfer radical reaction for the highly selective introduction of azido group into a wide range of prenylated molecules. Upon condition optimization, the fluorescent labeling of nucleic acids with prenyl modification in vitro have been achieved following this well-established protocol along with subsequent click reaction with DBCO-Cy5.(c) 2022 Elsevier Ltd. All rights reserved.

Automated summary

In this study, we utilized azidotrimethylsilane/Selectfluor via single electron transfer radical reaction to introduce azido group selectively into various prenylated molecules. After optimizing the conditions, we achieved fluorescent labeling of nucleic acids with prenyl modification in vitro using this established protocol, followed by click reaction with DBCO-Cy5.