Journal
TALANTA
Volume 241, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2022.123254
Keywords
Cancer antigen 125; Ordered mesoporous carbon; Ferrocenecarboxylic acid; Layer-by-layer assembly; Layered double hydroxides
Categories
Funding
- National Natural Science Foundation of China [81773480, 81973097]
Ask authors/readers for more resources
In this study, a label-free electrochemical immunosensor was constructed for the detection of cancer antigen 125 (CA125). This immunosensor used a multiple-enlargement means of layer-by-layer (LBL) assembly, incorporating ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs), and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). The LBL assembly approach greatly amplified the signal of the immunosensor and improved the immobilization of biomolecules.
A label-free electrochemical immunosensor was constructed for cancer antigen 125 (CA125) detection based on multiple-enlargement means of layer-by-layer (LBL) assembly of ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs) and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). A CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was designed using technology of LBL self-assembly among negatively charged Au NPs, positively charged CMK-3 and Fc@MgAl-LDH nanosheets. The CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was used as carriers to increase the immobilization of antibody and the number of loading Fc, conductors to strengthen conductivity and enhancers to amplify signal of Fc step-by-step. Besides, this special and excellent way of LBL assembly can immensely amplify the signal of immunosensor and more immobilize the biomolecules, and label-free method is a more simple the measuring way and the procedure. The immunosensor displayed a wider linear range of 0.01 U ml 1-1000 U ml(-1) and a lower detection limit of 0.004 U ml(-1). Therefore, the sensor can stablely and accurately be applied for CA125 detection in clinical cancer diagnosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available