Advanced detection and sensing strategies of Pseudomonas aeruginosa and quorum sensing biomarkers: A review

Pseudomonas aeruginosa (P. aeruginosa), a ubiquitous opportunistic pathogen, can frequently cause chronic obstructive pulmonary disease, cystic fibrosis and chronic wounds, and potentially lead to severe morbidity and mortality. Timely and adequate treatment of nosocomial infection in clinic depends on rapid detection and accurate identification of P. aeruginosa and its early-stage antibiotic susceptibility test. Traditional methods like plating culture, polymerase chain reaction, and enzyme-linked immune sorbent assays are time-consuming and require expensive equipment, limiting the rapid diagnostic application. Advanced sensing strategy capable of fast, sensitive and simple detection with low cost has therefore become highly desired in point of care testing (POCT) of nosocomial pathogens. Within this review, advanced detection and sensing strategies for P. aeruginosa cells along with associated quorum sensing (QS) molecules over the last ten years are discussed and summarized. Firstly, the principles of four commonly used sensing strategies including localized surface plasmon resonance (LSPR), surface-enhanced Raman spectroscopy (SERS), electrochemistry, and fluorescence are briefly over viewed. Then, the advancement of the above sensing techniques for P. aeruginosa cells and its QS biomarkers detection are introduced, respectively. In addition, the integration with novel compatible platforms towards clinical application is highlighted in each section. Finally, the current achievements are summarized along with proposed challenges and prospects.

Automated summary

This review discusses and summarizes advanced detection and sensing strategies for P. aeruginosa cells and its associated quorum sensing molecules over the past ten years. The aim is to achieve rapid, sensitive, and cost-effective diagnosis for infection. The principles of commonly used sensing strategies are briefly introduced, followed by the advancement of these techniques in detecting P. aeruginosa cells and quorum sensing biomarkers. Additionally, the integration of novel platforms for clinical application is highlighted. The current achievements are summarized, and challenges and prospects for the future are proposed.