Journal
TALANTA
Volume 249, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2022.123612
Keywords
Dual nanozyme; 2D conductive metal-organic framework nanosheets; Gold nanoparticles; Electrochemical biosensor; Cancer biomarker detection
Categories
Funding
- National Natural Science Foundation of China [51772110, 82102513]
- Internal Research Funding of Khalifa University of Science and Technology (KU) [CIRA-2018-16]
Ask authors/readers for more resources
In this study, a high-performance electrochemical biosensor based on a highly active dual nanozyme amplified system was developed for sensitive detection of the cancer biomarker H2O2 in live cells. The nanohybrid modified electrode exhibited good sensing performances with a low detection limit and high sensitivity. The biosensor was successfully applied for real-time tracking of H2O2 released from different human colon cells, showing great potential for early diagnosis and management of various cancer diseases.
The development of facile, rapid and cost-effective strategies for sensitive detection of cancer biomarkers in human samples is of great significance for early diagnosis of malignant tumors related diseases. In this work, we develop a high-performance electrochemical biosensor based on highly active dual nanozyme amplified system, i.e., ultrathin two-dimension (2D) conductive metal-organic framework (C-MOF) nanosheets (NSs) decorated with high-density ultrafine gold nanoparticles (Au-NPs), and explore its application in sensitive detection of cancer biomarker H2O2 in live cells. The C-MOF NSs {i.e., Cu-HHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene)-NSs} provide large surface area and abundant active open metal sites (Cu-O-4), which could improve the catalytic activity of Cu-HHTP-NSs towards H2O2. Moreover, abundant exposed O atoms also serve as anchor sites for the deposition of high-density ultrafine Au-NPs (~3 nm) without agglomeration. Owing to the synergistic contributions of high catalytic activity of Cu-HHTP-NSs and Au-NPs as well as their unique structural and electrical properties, the as-prepared nanohybrid modified electrode exhibits good sensing performances to H2O2 with an extremely low detection limit of 5.6 nM (3 sigma rules) and a high sensitivity of 188.1 mu A cm(-2) mM(-1). Furthermore, the proposed nanozymatic electrochemical biosensor has been applied in real-time tracking H2O2 released from different human colon cells to identify colon cancer cells from normal colon epithelial cell, which demonstrates its great prospect for early diagnosis and management of various cancer diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available