Journal
TALANTA
Volume 239, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.123129
Keywords
Mucin 1 (MUC1); Enzyme-free; Hybridization chain reaction (HCR); Detection; In situ imaging
Categories
Funding
- National Key R&D Project of China [2019YFC1520500]
- National Natural Science Foundation of China [81673399]
- Natural science Foundation of Zhejiang Province [LGC21H300004]
- Fundamental Research Funds for the Central Universities [2019FZA7017]
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In this study, a simple enzyme-free method for detecting and imaging MUC1 was developed. It was successfully used to detect MUC1 in specific cells and perform in situ imaging.
Mucin 1 (MUC1) overexpression in tumor cells is related to various cancers, including breast, stomach, and lung cancer. MUC1 detection and imaging are important for cancer localization in tissue sections to support histo-pathological diagnosis. In this study, we developed a simple, enzyme-free MUC1 detection and in situ imaging method. Three hairpin probes, Apt-trigger, HP1-FAM, and HP2, were designed for MUC1 recognition and hy-bridization chain reaction (HCR). The Apt-trigger probe was composed of two sequences: the MUC1 aptamer and HCR trigger sequence. The 5' end of the HP1-FAM probe was modified with a FAM signal molecule. In the presence of MUC1, the aptamer sequence is activated and bound to MUC1, which opens the hairpin structure. Then, the trigger sequence gets exposed and, complementary to HP1-FAM, triggers a continuous HCR process. This method was successfully used to detect MUC1 of 200 pM-25 nM and MUC1 in situ imaging in specific cells, such as human breast carcinoma (MCF-7) and human colon cancer (HT-29) cells.
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