Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 272, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.120903
Keywords
Cysteine; S-CDs; WS2 nanosheet; Au nanoparticles; Fluorescence resonance energy transfer; FRET
Categories
Funding
- Iran National Science Foundation [INSF 98016067]
- North Khorasan University of Medical Sciences [NKUMS 990206]
- Research Council of University of Tehran
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This article introduces a fluorescence resonance energy transfer (FRET) system for detecting cysteine, and optimizes the pH, temperature, and time to achieve good detection performance. The method can rapidly and sensitively detect the concentration of cysteine in human blood serum.
Cysteine is an essential biothiol that plays various functions in the human body. Decrease or exceeding of this excellent antioxidant from the expected range will lead to so many problems. Thus, appropriate sens-ing of it would be of great importance. Sulfur-doped carbon Dots (S-CDs) owe excellent fluorescence emission. Therefore, designing a Fluorescence resonance energy transfer (FRET) system between S-CDs as donor and Au nanoparticles (AuNPs) decorated tungsten disulfide nanosheet (WS2 NSs) would be a perfect strategy for cysteine detection. Excitation at 340 nm will give the maximum quantum yield of S-CDs (21%) and fluorescence emission peak at 460 nm. In the presence of cysteine, the FRET mechanism inhibited through the affinity of cysteine's functional groups (-SH and-NH2) toward AuNPs and S-CDs fluorescence emission was recovered. To find the best efficiency of the system, optimization of pH, tem-perature, and time was investigated. Here the linear range of 3-275 mM and limit of detection of 0.01 mM was obtained. Finally, the fluorescence method was applied to the analysis of cysteine in human blood serum, which poses the potential of rapid and sensitive sensing. It can detect both lower and higher amounts of serum cysteine. (c) 2022 Elsevier B.V. All rights reserved.
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