BF2 group chelated AIE fluorescent probe for polarity mapping of lipid droplets in cells and in vivo

Lipid droplets (LDs), are multi-functional organelles with the storage of neutral lipids and proteins, participating in various of physiological processes. However, abnormal of LDs in morphology and numbers always lead to multiple diseases, including cancer, viral infection, obesity, inflammation. To better understand the physiological function of LDs in living cells, we designed two new fluorescent probes LDs-CA and LDs-BCA based on the triphenylamine and coumarin fluorophores to monitor LDs polarity and numbers variation in this work. The one-step strategy for the regulation of BF2 group realized a gratifying in emission wavelengths from orange fluorescence of LDs-CA to the red fluorescence of LDs-BCA, surprisingly. The two novel probes showed strong positive solvatochromism effect in different solvents and exhibited the aggregation-induced emission (ALE) effect. Based on the above excellent optical properties, LDs-CA and LDs-BCA were applied for imaging of the LDs with high overlap coefficient when co-stained with commercial dyes, respectively. The probes of LDs-CA and LDs-BCA provided an intuitive method to visualize the dynamic changes of LDs in morphology, size, and numbers under nutritional stimulation, affording a powerful tool for fluorescence visualization of LDs related biological processes. Notably, the near-infrared emissive probe LDs-BCA successfully imaged the gastric fat in living obese mouse, which may provide a new idea for medical diagnostics. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The new fluorescent probes LDs-CA and LDs-BCA were designed to monitor the polarity and number variations of lipid droplets, showing strong positive solvatochromism effect and aggregation-induced emission effect in different solvents. These probes provided a powerful tool for fluorescence visualization of lipid droplets related biological processes and successfully imaged gastric fat in living obese mouse, suggesting a new idea for medical diagnostics.