Journal
SKIN PHARMACOLOGY AND PHYSIOLOGY
Volume 35, Issue 4, Pages 206-214Publisher
KARGER
DOI: 10.1159/000524188
Keywords
Adipose-derived stem cells; Sebaceous glands; Gelatin microspheres; Hepatocyte growth factor; 5a-dihydrotestosterone; Wound healing
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Funding
- National Natural Science Foundation of China [81871561]
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This study demonstrates the effectiveness of ADSCs treated with amnion and HGF- and 5 alpha-DHT-gelatin microspheres in promoting wound healing and restoring sebaceous glands.
Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of adipose-derived stem cells (ADSCs) into sebaceous glands and clarified the involvement of hepatocyte growth factor (HGF) and 5 alpha-dihydrotestosterone (5 alpha-DHT) in this process. Methods: This study used HGF- and 5 alpha-DHT-gelatin microspheres to treat human ADSCs and investigated the reconstruction of sebaceous glands. HGF- and 5 alpha-DHT-gelatin microspheres were constructed using microcapsule slow-release technology. A mice full-thickness skin-wound model was established to evaluate wound healing, and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF- and 5 alpha-DHT-gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial-cell growth, was activated after HGF and 5 alpha-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF- and 5 alpha-DHT-gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF- and 5 alpha-DHT-gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds. (C) 2022 S. Karger AG, Basel
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