Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 358, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131525
Keywords
Prussia blue nanoparticles; Alkaline phosphatase; Zearalenone; Dual-signal sensor
Funding
- National Key R&D Pro-gram of China [2018YFC1602500]
- National Natural Science Foundation of China [31871888]
- Special Project of Jilin Province School Co-Construction Plan of China [SXGJSF2017-6]
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A dual-signal immunoassay utilizing alkaline phosphatase was developed to detect Zearalenone (ZEN) in cornmeal. The method combines colorimetric and electrochemical detection, generating Prussian blue nanoparticles and multicolor changes. The results demonstrate a high sensitivity for ZEN detection in corn samples through UV-vis spectrometry and differential pulse voltammetry.
Zearalenone (ZEN), as one of the most important foods and feeds pollutant mycotoxins, is threatening human and animal health. Here, we have developed a dual-signal immunoassay triggered by alkaline phosphatase (ALP) for detecting ZEN in cornmeal. The immobilized ZEN-bovine serum albumin and free ZEN in the sample were competitively bound to an anti-ZEN monoclonal antibody (McAb). Then, the ALP labeled goat anti-mouse IgG (ALP-IgG) combined with the McAb. ALP catalyzed ascorbic acid 2-phosphate to produce L-ascorbic acid (AA). AA converted potassium ferricyanide (K-3[Fe(CN)(6)]) to potassium ferrocyanide (K-4[Fe(CN)(6)]), which reacted with ferric ion (III) to promote the formation of Prussian blue nanoparticles (PB NPs). Consequently, the solution appeared multicolor changes. Meanwhile, PB NPs had a maximum absorption peak at 700 nm. It could be monitored by an UV-vis spectrometer. As an electron transfer medium, K-3[Fe(CN)(6)] was consumed gradually along with PB NPs formation. Therefore, electrochemical detection was also used for detecting ZEN. Under the optimum conditions, the logarithm of ZEN concentration showed a good linear relationship with the absorbance from 0.2 to 0.8 ng/mL (R-2 = 0.987) and the differential pulse voltammetry peak current from 0.125 to 0.5 ng/mL (R-2 = 0.993). The limits of detection of colorimetric and electrochemical methods were 0.04 and 0.08 ng/mL, respectively. The recovery rates of ZEN from cornmeal samples were in the range of 80-120%, and relative standard deviations were lower than 10%. The results demonstrated that the dual-signal immunoassay has a high sensitivity for ZEN detection in corn samples.
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