Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 358, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131505
Keywords
Near-infrared; Ratiometric; Alkaline phosphatase; Fluorescent probe; Imaging
Funding
- National Natural Science Foundation of China [21772078, 22071200]
- Fundamental Research Funds for the Central Universities [2682020CX55, 2682021ZTPY011]
- MoE of China [XJ2021KJZK004]
- Science and Technology Department of Sichuan Province, China [2020JDRC0021]
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The newly designed NIR fluorescent probe SWJT-3 is capable of ratiometric determination of alkaline phosphatase (ALP) and can be used for both in vitro and in vivo detection. It is the first NIR and ratiometric fluorescent probe for imaging ALP in mice, with a large Stokes shift and low detection limit.
A novel near-infrared (NIR) fluorescent probe (SWJT-3) was designed based on phosphate protection of the electron donating group of the hemicyanine for the ratiometric determination of ALP at 590 and 670 nm. Notably, SWJT-3 could be used in the endogenous detection of ALP in vitro and vivo. Moreover, it was the first NIR and ratiometric fluorescent probe for the imaging of ALP in mice. It has large Stokes shift (185 nm), and also showed high selectivity, low detection limit (0.87 U/L). Meanwhile, SWJT-3 exhibited obvious color change in aqueous solution which could be observed by naked-eyes. In addition, the probe displayed low lifetime (0.74 ns), high substrate affinity (K-m = 8.89 mu M) and excellent fluorescence characterization under various polarity and viscosity conditions.& nbsp;
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