A Microwave-Based Microfluidic Cell Detecting Biosensor for Biological Quantification Using the Metallic Nanowire-Filled Membrane Technology

A label-free, sensitive, miniaturized sensing device was developed for detecting living cells in their flow stream. The outstanding performance of this biosensor in distinguishing living cells in cell suspension was achieved by integrating microstrip stub resonator above a microfluidic structure using the metallic nanowire-filled membrane technology. The cell suspension flows in a microfluidic channel placed between the tip of the stub resonator and its ground plane as the substrate to take advantage of the uniform and concentrated field distribution. We studied the changes in relative permittivity due to the presence of a single living cell in the phase of the transmitted signal (S-21). An average variation of as much as 22.85 +/- 1.65 degrees at similar to 11.1 GHz is observed for the living cell sensing using this optimized device. This biosensor could detect rapid flowing cells in their biological medium in real-time and hence, can be used as an early diagnosis and monitoring tool for diseases.

Automated summary

A label-free, sensitive, miniaturized sensing device has been developed for detecting living cells in their flow stream. Through the integration of a microstrip stub resonator above a microfluidic structure using metallic nanowire-filled membrane technology, this biosensor demonstrates outstanding performance in distinguishing living cells in cell suspension. The device takes advantage of the uniform and concentrated field distribution between the tip of the stub resonator and its ground plane as the substrate, where the cell suspension flows in a microfluidic channel. By studying the changes in relative permittivity caused by the presence of a single living cell in the phase of the transmitted signal (S-21), an average variation of as much as 22.85 +/- 1.65 degrees at approximately 11.1 GHz is observed for living cell sensing with this optimized device. This biosensor can detect rapidly flowing cells in their biological medium in real-time, making it a potential tool for early disease diagnosis and monitoring.