The salicylic acid mediates selenium-induced tolerance to drought stress in tomato plants

The role of selenium (Se) in mitigating detrimental effects of drought stress on the plants has been reported, but the mechanism is still unclear. In this study, the function of Se fertilization on tomato (Solanum lycopersicum L.) under drought stress was characterized. Our results showed that the growth reduction caused by drought stress was alleviated by application of exogenous Se to a large extent. In addition, the treatment of Se significantly increased the net photosynthetic rate (Pn), transpirational rate (Tr), stomatal conductance (Gs) and water use efficiency (WUE) than in the absence of Se under drought stress treatment. Se addition also significantly inhibited the increase of malondialdehyde (MDA), soluble sugar and proline levels of tomato leaves and produced less reactive oxygen species (ROS) accumulation under drought stress. Furthermore, Se supplementation to the nutrient solution significantly increased the endogenous salicylic acid (SA) and methylated SA (MeSA) levels in tomato roots and promoted the conversion from SA to MeSA in tomato leaves under drought stress conditions via regulating the expression levels of several genes involved in SA biosynthesis and conversion such as PAL1, ICS, SAMT and SABP2. But the pretreatment with ABT (1-aminobenzotriazole, a SA inhibitor) abolished the beneficial effect of Se on drought stress tolerance of plants. These results suggested that Se application promoted photosynthetic efficiency and plant growth, alleviated drought-induced oxidative stress, and increased the endogenous SA levels, thereby imparting drought stress tolerance of tomato plants. This study may provide a theoretical basis for utilization of Se fertilizer to improve the yield and quality of agricultural products under stress.

Automated summary

This study found that the application of selenium can alleviate growth reduction, improve photosynthetic efficiency and plant growth, and reduce drought-induced oxidative stress in tomatoes. Selenium treatment also increased the levels of endogenous salicylic acid and methylated salicylic acid, and promoted the conversion from salicylic acid to methylated salicylic acid. This research provides a theoretical basis for using selenium fertilizer to improve the yield and quality of agricultural products under stress conditions.