Alteration in microbial community and antibiotic resistance genes mediated by microplastics during wastewater ultraviolet disinfection

Microplastics (MPs) could be as a vector to colonize microorganisms and antibiotic resistance gene (ARGs) in surface water. However, little information is known regarding their changes by the presence of MPs in wastewater treatment. Here, the effects of different concentrations and sizes of polystyrene microplastics (PSMPs) on the distribution and removal of microbial communities and ARGs under ultraviolet disinfection of urban sewage have been systematically studied. Results showed that the presence of MPs altered abundance and functions of microorganisms in wastewater, despite different effects on different types of microorganisms. The most abundant ARGs in original disinfection tank sewage was rpoB2 (6.34%). A certain concentration range of MPs can improve the ability of specific types of ARGs in the UV disinfection process. Compared to the system without PSMPs, the content of Deinococcus-Thermus and Bacteroidetes phylum increased, while Actinobacteria and Proteobacteria phylum decreased in the presence of MPs. The microbial functions, especially the genetic information processing and metabolism were altered by the presence of PSMPs. In addition, PSMPs altered the content of ARGs, where the contents of OXA-182 and ErmH were increased, while adeF and ANT3-Iic were decreased. PSMPs also decreased the free ARB content in wastewater by providing colonization sites. The UV disinfection efficiency of microorganisms and ARGs was also intervened by PSMPs since they provided colonization sites and increased the water turbidity. The findings indicated that PSMPs altered the distribution and removal of microbial community and ARGs in ultraviolet disinfection of wastewater, highlighting the combined risks.

Automated summary

The presence of microplastics in wastewater alters the abundance and functions of microorganisms, as well as the distribution and removal of antibiotic resistance genes (ARGs). The microbial community and ARGs are affected differently by the presence of microplastics. Certain concentrations of microplastics can enhance the ability of specific types of ARGs during ultraviolet disinfection. PSMPs change the content of microbial phyla and shift microbial functions. They also alter the content of ARGs, increasing some ARGs while decreasing others. PSMPs decrease the free ARG content in wastewater and intervene the efficiency of UV disinfection. Overall, the presence of PSMPs alters the distribution and removal of microbial community and ARGs, highlighting the combined risks.