Genomic features of a multidrug-resistant and mercury-tolerant environmental Escherichia coli recovered after a mining dam disaster in South America

Mining dam disasters contribute to the contamination of aquatic environments, impacting associated ecosystems and wildlife. A multidrug-resistant Escherichia coli strain (B2C) was isolated from a river water sample in Brazil after the Mariana mining dam disaster. The genome was sequenced using the Illumina MiSeq platform, and de novo assembled using Unicycler. Resistome, virulome, and plasmidome were predicted using bioinformatics tools. Data analysis revealed that E. coli B2C belonged to sequence type ST219 and phylogroup E. Strikingly, a broad resistome (antibiotics, hazardous heavy metals, and biocides) was predicted, including the presence of the clinically relevant blaCTX-M-2 extended-spectrum j3-lactamase (ESBL) gene, qacE Delta 1 efflux pump gene, and the mer (mercury resistance) operon. SNP-based analysis revealed that environmental E. coli B2C was clustered along to ESBL-negative E. coli strains of ST219 isolated between 1980 and 2021 from livestock in the United States of America. Acquisition of clinically relevant genes by ST219 seems to be a recent genetic event related to anthropogenic activities, where polluted water environments may contribute to its dissemination at the human-animal-environment interface. In addition, the presence of genes conferring resistance to heavy metals could be related to environmental pollution from mining activities. Antimicrobial resistance genes could be essential biomarkers of environmental exposure to human and mining pollution.

Automated summary

Mining dam disasters can cause contamination in aquatic environments, affecting ecosystems and wildlife. In the aftermath of the Mariana mining dam disaster in Brazil, a multidrug-resistant Escherichia coli strain (B2C) was found in a river water sample. This strain exhibited a broad resistome, including clinically relevant resistance genes. Analysis showed that this strain clustered with ESBL-negative E. coli strains from livestock in the United States, indicating recent acquisition of clinically relevant genes related to human activities. Presence of heavy metal resistance genes may be linked to environmental pollution from mining activities.