4.7 Article

Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR

Related references

Note: Only part of the references are listed.
Article Environmental Sciences

Detection of SARS-CoV-2 in raw and treated wastewater in Germany - Suitability for COVID-19 surveillance and potential transmission risks

Sandra Westhaus et al.

Summary: Wastewater-based monitoring showed the presence of SARS-CoV-2 genetic traces in different raw wastewaters in North Rhine-Westphalia, Germany. However, it was suggested that wastewater might not be a major route for transmission to humans. Further validation for enveloped viruses in wastewater is needed.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Environmental Sciences

Detection of SARS-CoV-2 RNA in bivalve mollusks and marine sediments

David Polo et al.

Summary: This study utilized bivalve molluscan species and estuarine sediments as samples to detect the presence of SARS-CoV-2 RNA traces in the estuarine environment through RT-qPCR and PMAxx-Triton viability RT-qPCR assays.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Engineering, Environmental

Several forms of SARS-CoV-2 RNA can be detected in wastewaters: Implication for wastewater-based epidemiology and risk assessment

S. Wurtzer et al.

Summary: SARS-CoV-2 virus can be detected not only in respiratory droplets but also in human feces, urine, and raw wastewaters, suggesting possible alternative routes of infection. Monitoring virus genomes in wastewater can complement tracking virus transmission within the human population.

WATER RESEARCH (2021)

Article Multidisciplinary Sciences

Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples

Enric Cuevas-Ferrando et al.

Summary: This research aimed to develop a rapid viability RT-qPCR procedure to detect potentially infectious SARS-CoV-2 particles in clinical specimens and environmental samples. Using genomic RNA and various samples for validation, it was shown that this method can identify infectious viruses in complex matrices.

SCIENTIFIC REPORTS (2021)

Review Engineering, Environmental

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review

Mats Leifels et al.

Summary: Capsid integrity quantitative PCR (qPCR) is a molecular detection method for infectious viruses, but variations in pre-treatment conditions for different virus types may limit the efficacy of specific protocols. It is recommended to optimize specific protocols for each virus and a framework for general assay conditions has been identified, which can benefit the investigation of infectious virus transmission.

WATER RESEARCH X (2021)

Article Infectious Diseases

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman et al.

EUROSURVEILLANCE (2020)

Editorial Material Gastroenterology & Hepatology

Evidence for Gastrointestinal Infection of SARS-CoV-2

Fei Xiao et al.

GASTROENTEROLOGY (2020)

Article Medicine, General & Internal

First Case of 2019 Novel Coronavirus in the United States

Michelle L. Holshue et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Biochemistry & Molecular Biology

Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses

Furong Qi et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

Virological assessment of hospitalized patients with COVID-2019

Roman Woelfel et al.

NATURE (2020)

Letter Critical Care Medicine

Single-Cell RNA Expression Profiling of ACE2, the Receptor of SARS-CoV-2

Yu Zhao et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2020)

Editorial Material Medicine, General & Internal

Airborne Transmission of SARS-CoV-2 Theoretical Considerations and Available Evidence

Michael Klompas et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2020)

Article Engineering, Environmental

SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area

Walter Randazzo et al.

WATER RESEARCH (2020)

Article Engineering, Environmental

COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology

Raul Gonzalez et al.

WATER RESEARCH (2020)

Article Engineering, Environmental

Persistence of SARS-CoV-2 in Water and Wastewater

Aaron Bivins et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS (2020)

Article Public, Environmental & Occupational Health

Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients from the Republic of Korea

Jeong-Min Kim et al.

OSONG PUBLIC HEALTH AND RESEARCH PERSPECTIVES (2020)

Letter Immunology

Infectious SARS-CoV-2 in Feces of Patient with Severe COVID-19

Fei Xiao et al.

EMERGING INFECTIOUS DISEASES (2020)

Article Environmental Sciences

Glass Wool Concentration Optimization for the Detection of Enveloped and Non-enveloped Waterborne Viruses

Albert Blanco et al.

FOOD AND ENVIRONMENTAL VIROLOGY (2019)

Article Engineering, Environmental

Evaluation of Phi6 Persistence and Suitability as an Enveloped Virus Surrogate

Nathalia Aquino de Carvalho et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2017)

Article Engineering, Environmental

Survivability, Partitioning, and Recovery of Enveloped Viruses in Untreated Municipal Wastewater

Yinyin Ye et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2016)

Article Public, Environmental & Occupational Health

Use of ethidium monoazide and propidium monoazide to determine viral infectivity upon inactivation by heat, UV- exposure and chlorine

Mats Leifels et al.

INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH (2015)

Article Biochemical Research Methods

Propidium monoazide reverse transcriptase PCR and RT-qPCR for detecting infectious enterovirus and norovirus

Mohammad R. Karim et al.

JOURNAL OF VIROLOGICAL METHODS (2015)

Article Biochemical Research Methods

Measurement of the virolysis of human GII.4 norovirus in response to disinfectants and sanitisers

P. Nowak et al.

JOURNAL OF VIROLOGICAL METHODS (2011)

Article Environmental Sciences

Survival of Coronaviruses in Water and Wastewater

Patricia M. Gundy et al.

FOOD AND ENVIRONMENTAL VIROLOGY (2009)

Article Biotechnology & Applied Microbiology

Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification

Safaa Lamhoujeb et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2008)

Review Engineering, Environmental

Review of factors affecting microbial survival in groundwater

DE John et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2005)