Role of extracellular polymeric substances in the immobilization of hexavalent chromium by Shewanella putrefaciens CN32 unsaturated biofilms

Microbial remediation provides a promising avenue for the management and restoration of heavy metal-contaminated soils. Microorganisms in soils usually exist within unsaturated biofilms, however, their response to heavy metals is still limited compared to saturated biofilms. This work investigated the Cr(VI) immobilization by Shewanella putrefaciens CN32 unsaturated biofilms, and explored the underlying mechanisms of Cr(VI) com-plexation. Results reveal a dose-dependent toxicity of Cr(VI) to the growth of the unsaturated biofilms. During the early growth stage, the Cr(VI) addition stimulated more extracellular polymeric substances (EPS) production. In the meantime, the EPS were demonstrated to be the primary components for Cr(VI) immobilization, which accounted for more than 60% of the total adsorbed Cr(VI). The Fourier transform infrared spectra and X-ray pho-toelectron spectra corroborated that the binding sites for immobilizing Cr(VI) were hydroxyl, carboxyl, phospho-ryl and amino functional groups of the proteins and polysaccharides in EPS. However, for the starved unsaturated biofilms, EPS were depleted and the EPS-bound Cr(VI) were released, which caused approximately 60% of the adsorbed Cr(VI) onto cell components and further aggravated the Cr(VI) stress to cells. This work extends our un-derstanding about the Cr(VI) immobilization by unsaturated biofilms, and provides useful information for reme-diation of heavy metal-contaminated soils. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

Microbial remediation is a promising approach for managing and restoring heavy metal-contaminated soils. This study investigated the immobilization of Cr(VI) by Shewanella putrefaciens CN32 unsaturated biofilms and explored the underlying mechanisms of Cr(VI) complexation. The results showed that Cr(VI) had a dose-dependent toxicity to the growth of unsaturated biofilms. The extracellular polymeric substances (EPS) were found to be the primary components for Cr(VI) immobilization, accounting for over 60% of the total adsorbed Cr(VI). The study also revealed that hydroxyl, carboxyl, phosphoryl, and amino functional groups in proteins and polysaccharides of EPS were the binding sites for immobilizing Cr(VI). Starved unsaturated biofilms released the EPS-bound Cr(VI) due to the depletion of EPS, which further aggravated the toxicity of Cr(VI) to the cells. This study expands our understanding of Cr(VI) immobilization by unsaturated biofilms and provides valuable information for the remediation of heavy metal-contaminated soils.