4.7 Article

Intrauterine hyperglycemia impairs endometrial receptivity via up-regulating SGK1 in diabetes

Journal

SCIENCE CHINA-LIFE SCIENCES
Volume 65, Issue 8, Pages 1578-1589

Publisher

SCIENCE PRESS
DOI: 10.1007/s11427-021-2035-2

Keywords

diabetes; endometrial receptivity; hyperglycemia; serum; glucocorticoid-regulated kinase

Categories

Funding

  1. National Natural Science Foundation of China [81490742, 82001537]
  2. Fundamental Research Funds for the Central Universities [2021FZZX003-02-18]
  3. National Key Research and Development Program of China [2018YFC1004402]

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Diabetes can adversely affect reproductive function and lead to abnormal SGK1 expression in the endometrium. Enhanced expression of SGK1 in a high-glucose environment further reduces embryo implantation and causes aberrant expression of LIF and MUC1.
Diabetes is a complex metabolic disorder which can adversely affect reproductive function. SGK1 is found to be up-regulated in multiple tissues of diabetic patients. However, the effects of diabetes on endometrial SGK1 expression and endometrial receptivity remain unknown. In this study, we established a streptozotocin-induced diabetic mouse model and observed reduced implantation sites, retarded development of pinopodes, increased SGK1, and aberrant expression of LIF and MUC1 in the endometrial epithelium. We injected the uterine lumen of normal mice with high-glucose solution and cultured endometrial cells in high-glucose medium to mimic intrauterine hyperglycemia. Both studies provided compelling evidence that hyperglycemia could lead to diminished embryo implantation and dysregulated SGK1, LIF and MUC1. Additionally, through over-expression of SGK1 in vivo and in vitro, we found that enhanced SGK1 also decreased LIF expression, increased MUC1 expression, and attenuated embryo implantation rate. We further identified that hyperglycemia-activated SMAD2/3 might be responsible for the enhancement of SGK1 and verified directly the interaction between SMAD3 and corresponding SMAD binding elements within SGK1 promoter. Taken together, our study confirmed the association between diabetes-related hyperglycemia and endometrial receptivity defects. Hyperglycemia-induced SGK1 has a tremendous role in this pathological process, rendering it as an attractive therapeutic target for diabetes-related reproductive disorders.

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