Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 53, Issue 3, Pages 231-238Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2022.2073597
Keywords
Bioethanol; dual pretreatment; rice straw; saccharomyces; sequential pretreatment
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Sequential pretreatment using different NaOH and H2SO4 concentrations was evaluated for efficient hydrolysis of rice straw. The biomass pretreated with sequential H2SO4 and NaOH achieved higher sugar yield compared to sequential NaOH and H2SO4 pretreatment. Increasing NaOH concentration improved the sugar yields in both pretreatment methods.
Sequential pretreatment using different NaOH concentrations (0.5%, 1.0%, 1.5%, w/w) and 1% H2SO4 (w/w) was evaluated as a strategy for effective hydrolysis of rice straw. The efficiency of sequential NaOH and H2SO4 (SNA) pretreatment against sequential H2SO4 and NaOH (SH) was assessed. SH pretreated biomass attained more sugar yield compared to SNA pretreated biomass. The sugar yields from pretreated biomass improved with increasing NaOH concentration in both SH and SNA treatments. The maximum sugar release of 40.6 mg/ml (83.2% efficiency) was obtained from SH pretreated biomass when the stage 2 alkali treatment was performed at 1.5% w/w NaOH. The non-detoxified hydrolysate from this biomass was fermented with 96.8% efficiency.
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