Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 53, Issue 2, Pages 157-166Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2022.2053991
Keywords
Cyclodepsipeptide; elicitor; response surface methodology; Streptomyces sp; valinomycin
Ask authors/readers for more resources
In this study, the production of valinomycin by Streptomyces sp. ZJUT-IFE-354 was significantly increased by optimizing the medium composition and culture conditions, resulting in the highest reported yield. This provides an efficient method for enhancing valinomycin production.
Valinomycin is a cyclodepsipeptide antibiotic with a broad spectrum of biological activities, such as antiviral, antitumor, and antifungal activities. However, the low yield of valinomycin often limits its applications in medicine, agriculture, and industry. In our previous report, Streptomyces sp. ZJUT-IFE-354 was identified as a high-yielding strain of valinomycin. In this study, Plackett-Burman design (PBD) and response surface methodology (RSM) were used to optimize components of medium. The optimal medium contained 31 g/L glucose, 22 g/L soybean meal, and 1.6 g/L K2HPO4 center dot 3H(2)O, which could generate 262.47 +/- 4.28 mg/L of valinomycin. Then, the culture conditions were optimized by a one-factor-at-a-time (OFAT) approach. The optimal conditions for the strain included a seed age of 24 h, an inoculum size of 8% (v/v), an incubation temperature of 28 degrees C, an initial pH of 7.2, an elicitor of 0.1% Bacillus cereus feeding at 24 h cultivation, and the feeding of 0.6% (L)-valine at 36 h cultivation. The final valinomycin production increased to 457.23 +/- 9.52 mg/L, which was the highest yield ever reported. It highlights that RSM and OFAT may be efficient methods to enhance valinomycin production by Streptomyces sp. ZJUT-IFE-354.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available