Journal
PLATELETS
Volume 33, Issue 8, Pages 1139-1145Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/09537104.2022.2052035
Keywords
Flow cytometry; forward scatter; inherited platelet function disorder; inherited platelet number disorder; mean platelet diameter; platelets
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Funding
- St Vincent's Clinic Foundation Annual Research Grant
- St Vincent's Centre for Applied Medical Research Translational Research Grant
- Thrombosis and Hemostasis Society of Australia and New Zealand (THANZ) Educational
- Hemophilia Foundation of Australia Research Grant
- Pathology North (NSW Health Pathology) NGS support grant
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This study examined the feasibility of using platelet forward scatter (FSC) measurements to assess platelet size and its potential as an alternative to mean platelet diameter (MPD) or mean platelet volume (MPV). The results showed that platelet FSC correlated significantly with MPD and MPV, and could be used as an alternative measurement in cases where MPV is not available, such as macrothrombocytopenia.
The use of mean platelet diameter (MPD) to classify inherited thrombocytopenia (IT) has been demonstrated in several studies. Alternatively, the mean platelet volume (MPV) may be used, but in macrothrombocytopenia this may not be available. We hypothesized that platelet forward scatter (FSC) measurements using flow cytometry may be used for the size-based classification of IT. The study aimed to assess the ability of platelet FSC to measure platelet size and whether it could be used as an alternative to the MPD or MPV. Blood samples were obtained from individuals undergoing investigation for inherited platelet function disorders (IPFD, n = 40) or platelet number disorders (IPND, n = 46). A hematology analyzer was used to obtain MPV and platelet counts, flow cytometry to measure platelet FSC and ImageJ software to measure MPD from stained blood smears. The International Society of Thrombosis and Hemostasis (ISTH) Bleeding Assessment Tool (BAT) was used to calculate bleeding scores. Twenty-nine(63%) of IPND patients had an MPV that could not be reported. A significant correlation to platelet FSC was found to the MPD (p < .0001) and MPV (p < .0001) and an inverse correlation with platelet count (p < .0001). No significant correlation was found between FSC and bleeding history. In conclusion, platelet FSC is an alternative to MPV and may be used in macrothrombocytopenia where the MPV is not recorded
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