Journal
PLANT SCIENCE
Volume 318, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2022.111241
Keywords
Plant RNA; RNA degradome sequencing; RNA degradation
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Funding
- New Energy and Industrial Technology Development Organization (NEDO), Japan [16100893-0]
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This review discusses the findings and limitations of RNA degradation sequencing methods, and suggests further experiments needed to accurately understand RNA degradation intermediates. It provides direction for future research on RNA degradation and serves as a reference for RNA degradome studies in other species.
RNA degradation is an important process for controlling gene expression and is mediated by decapping / deadenylation-dependent or endonucleolytic cleavage-dependent RNA degradation mechanisms. High throughput sequencing of RNA degradation intermediates was initially developed in Arabidopsis thaliana and similar RNA degradome sequencing methods were conducted in other eukaryotes. However, interpreting results obtained by these sequencing methods is fragmented, and an overview is needed. Here we review the findings and limitations of these sequencing methods and discuss the missing experiments needed to understand RNA degradation intermediates accurately. This review provides direction for future research on RNA degradation and is a reference for RNA degradome studies in other species.
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