Hydrogen sulfide inhibits the abscission of tomato pedicel through reconstruction of a basipetal auxin gradient

Abscission is an important developmental process and an essential agricultural trait. Auxin and ethylene are two phytohormones with important roles in the complex, but still elusive signaling network of abscission. Here, we found that hydrogen sulfide (H2S), a newly identified gasotransmitter, inhibits the initiation of tomato pedicel abscission. The underlying mechanism was explored through transcriptome profile analysis in various pedicel tissues with or without H2S treatment in the early abscission stage. The data suggested that H2S strongly influences the global transcription of pedicel tissues, exerts differential expression regulation along the pedicel, and markedly influences both the auxin and ethylene signaling pathways. Computational analysis revealed that H2S reconstructs a basipetal auxin gradient along the pedicel at 4 h after treatment; this finding was further substantiated by the GUS-staining results of DR5::GUS pedicels. The inhibitory effect of H2S to the ethylene signaling pathway might be an indirect action. Moreover, the subtilisin-like proteinase family members involved in the release of peptide signal molecules are critical components of the abscission signaling network downstream of auxin and ethylene.

Automated summary

This study found that hydrogen sulfide inhibits the initiation of tomato pedicel abscission. Transcriptome analysis revealed that hydrogen sulfide strongly influences the global transcription of pedicel tissues and significantly affects both auxin and ethylene signaling pathways. Furthermore, hydrogen sulfide was found to reconstruct an auxin gradient along the pedicel and have an indirect inhibitory effect on the ethylene signaling pathway. In addition, the subtilisin-like proteinase family members were identified as critical components of the abscission signaling network downstream of auxin and ethylene.