Heritable base-editing in Arabidopsis using RNA viral vectors

Letter Plant Sciences

High-efficiency multiplex biallelic heritable editing in Arabidopsis using an RNA virus

Ugrappa Nagalakshmi et al.

PLANT PHYSIOLOGY (2022)

Article Biochemical Research Methods

Heritable gene editing using FT mobile guide RNAs and DNA viruses

Jianfeng Lei et al.

Summary: The virus-induced genome editing (VIGE) system has great application potential, but it cannot transfer edited genes to the next generation due to the inability of viruses to enter shoot apical meristem (SAM) in plants. A novel CLCrV-mediated VIGE system using the FT strategy enables heritable mutant offspring without tissue culture in A. thaliana.

PLANT METHODS (2021)

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Article Biochemistry & Molecular Biology

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

Tingdong Li et al.

Summary: This study developed a Barley stripe mosaic virus-based sgRNA delivery vector for heritable genome editing in Cas9-transgenic wheat plants, achieving mutated progenies with frequencies ranging from 12.9% to 100%. The researchers also successfully performed multiplex mutagenesis in progeny using a pool of BSMV-sg vectors, as well as devised a virus-induced transgene-free editing procedure to generate Cas9-free wheat mutants.

MOLECULAR PLANT (2021)

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Article Biotechnology & Applied Microbiology

Improved plant cytosine base editors with high editing activity, purity, and specificity

Qiurong Ren et al.

Summary: In this study, the A3A/Y130F-CBE_V01 was identified as the most efficient C-to-T base editor in plants, showing applications in generating microRNA-resistant mRNA transcripts and herbicide-resistant allele evolution in rice. Additionally, the development and testing of multiple CBE_V02, CBE_V03, and CBE_V04 systems in rice protoplasts demonstrated improved editing activity and purity, with the CBE_V04 systems showing enhanced editing efficiency through focal recruitment of uracil DNA glycosylase inhibitors by engineered sgRNA scaffolds. Whole-genome sequencing revealed varying levels of off-target effects among different CBE systems, with PmCDA1-CBE_V04 and A3A/Y130F-CBE_V04 identified as high-efficiency, high-purity, and highly specific next-generation plant CBEs.

PLANT BIOTECHNOLOGY JOURNAL (2021)

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Article Biotechnology & Applied Microbiology