Journal
PLANT JOURNAL
Volume 110, Issue 4, Pages 1021-1034Publisher
WILEY
DOI: 10.1111/tpj.15720
Keywords
apple; anthocyanin; MdMYB306-like; R2R3-MYB repressors; MdMYB17; MdbHLH33
Categories
Funding
- National Key Research and Development Program of China [2018YFD1000105]
- Agricultural Improved Seed Project of Shandong Province [2019LZGC008]
- National Natural Science Foundation of China [31872940]
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In this study, a MYB306-like anthocyanin repressor gene from apple was characterized and its significance in anthocyanin regulation was demonstrated. The MdMYB306-like protein can activate the expression of an anthocyanin repressor gene and inhibit the expression of an anthocyanin structural gene by directly binding to their promoters. The MdMYB306-like protein interacts with other transcription factors to enhance its regulatory activities on specific genes involved in anthocyanin synthesis.
Anthocyanin is one of the most important pigments and nutrients in fruits. Genes encoding R2R3-MYB transcription factors (TFs) are key to anthocyanin regulation. R2R3-MYB activators have been widely studied in the anthocyanin synthesis pathway. However, the mechanism by which R2R3-MYB repressors negatively regulate anthocyanin synthesis remains poorly understood. In the present study, we characterized a subfamily 1 R2R3-MYB anthocyanin repressor gene, MYB306-like, from apple (Malus x domestica) and demonstrated its significance in anthocyanin regulation. The MdMYB306-like protein activates the expression of an anthocyanin repressor gene, MdMYB17, and inhibits the expression of MdDFR (an anthocyanin structural gene) by directly binding to the corresponding promoters. The MdMYB306-like protein interacts with MdMYB17 and MdbHLH33 through its N-terminus. In addition, MdMYB306-like interacts with MdbHLH33 and MdMYB17 to enhance its regulatory activities on MdMYB17 and MdDFR, respectively. Our results revealed that the MdMYB306-like protein, in conjunction with MdMYB17 and MdbHLH33, forms a key regulatory module to fine-tune anthocyanin synthesis in apple.
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