Journal
PLANT JOURNAL
Volume 110, Issue 5, Pages 1493-1497Publisher
WILEY
DOI: 10.1111/tpj.15752
Keywords
UDP-glucose; cellulose; sucrose synthase; Arabidopsis thaliana
Categories
Funding
- Bio4Energy (Swedish Programme for Renewable Energy)
- Berzelii Centre for Forest Biotechnology - VINNOVA
- Swedish Research Council for Sustainable Development (Formas)
- Umea Plant Science Centre
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The role of SUS in cellulose biosynthesis in Arabidopsis has been refuted, as alternative UDP-glucose biosynthesis pathways were found to be employed.
Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.
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