Journal
PLANT DISEASE
Volume 106, Issue 10, Pages 2631-2637Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS-08-21-1836-RE
Keywords
brown spot; fungicide resistance; strobilurin
Categories
Funding
- United Soybean Board
Ask authors/readers for more resources
Brown spot, a common foliar disease of soybean caused by Septoria glycines, has developed resistance to QoI fungicides. By analyzing the cytochrome b gene, researchers discovered a mutation at codon 143 that is associated with QoI fungicide resistance. A PCR assay was successfully developed to distinguish between sensitive and resistant isolates. The study found that 47.5% of S. glycines isolates tested were resistant to QoI fungicides.
Brown spot, caused by Septoria glycines, is a common foliar disease of soybean (Glycine max). Applications of fungicide products that contain quinone outside inhibitor (QoI) active ingredients to soybean fields have contributed to the selection and development of QoI-resistant populations of S. glycines. We investigated the molecular mechanisms of QoI-resistance in these populations through targeted analysis of the cytochrome b gene. Isolates of S. glycines collected from several soybean fields over different seasons varied in sensitivity to QoI fungicides. Characterization of the cytochrome b gene revealed a mutation that changed an amino acid from glycine to alanine at codon 143 - one that is generally associated with QoI fungicide resistances. A PCR assay was developed that allowed successful discrimination of QoI-sensitive and -resistant isolates based on the G143A mutation. Results of this study demonstrated that 47.5% of S. glycines isolates tested were resistant to QoI fungicides. Accurate monitoring of this mutation will help slow the spread of QoI resistance and will be important for fungicide resistant management in this pathosystem.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available