Luteolin inhibits FcεRI- and MRGPRX2-mediated mast cell activation by regulating calcium signaling pathways

Luteolin is a flavonoid found in many fruits, vegetables, and herbs. The antiinflammatory effects of luteolin have been reported. In this study, the effect of luteolin on allergic diseases and the underlying molecular mechanism were investigated. We found that luteolin inhibits Fc epsilon RI (Fc epsilon RI)- and Mas-related G protein-coupled receptor X2 (MRGPRX2)-mediated mast cells (MCs) activation, including degranulation and release of cytokines in vitro. Moreover, luteolin reduces the degree of swelling and Evans blue exudation of mice paw in a dose-dependent manner. The concentrations of histamine, TNF-alpha, MCP-1, IL-8, and IL-13 in mice serum are also decreased by luteolin administration. Our study reveals that luteolin can inhibit Fc epsilon RI- and MRGPRX2-mediated allergic responses in vivo and in vitro, and our results discover luteolin inhibited mast cells mediated anaphylactic reaction by inhibiting the phosphorylation level of PLC gamma.

Automated summary

This study investigated the effect of luteolin on allergic diseases and found that it inhibits mast cell activation and release of cytokines. Luteolin also reduces swelling and exudation in mice and decreases the levels of inflammatory markers in serum.