Cassane-type diterpenes from roots of Pterolobium macropterum and their anti-inflammatory activity

Eight undescribed cassane diterpenes, pterolobirins C-J, together with two known analogs, were isolated from the roots of Pterolobium macropterum. Their structures were characterized by extensive spectroscopic techniques including NMR, MS, ECD and X-ray crystallographic spectroscopy. The absolute configuration of pterolobirin J was confirmed by single-crystal X-ray diffraction data. The compounds were screened for their anti-inflammatory activity on the lipopolysaccharide (LPS) induced nitric oxide (NO) production in J774. A1 macrophage cells. Pterolobirin E and sucutinirane C displayed good NO inhibition with IC50 values of 24.44 +/- 1.34 and 19.16 +/- 1.22 mu M, respectively.

Automated summary

Eight new cassane diterpenes, pterolobirins C-J, along with two known analogs, were isolated from the roots of Pterolobium macropterum. The structure of pterolobirin J was confirmed by X-ray diffraction analysis. Pterolobirin E and sucutinirane C exhibited significant inhibition of nitric oxide production.