Journal
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
Volume 118, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pmpp.2022.101807
Keywords
Amorphophallus muelleri; Soft rot disease; Pectobacterium carotovorum subsp. carotovorum; Comparative transcriptomics; Resistance genes
Categories
Funding
- National Natural Science Foun-dation of China [31660426]
- Yunnan Provincial Department of Science and Technology [2017FH001-005, 2018HB100, 2017FH001-035, 2019FH001-008]
- Yunnan Provincial Department of Education [2021J0707]
- Ministry of Education of the People's Republic of China [2019-NYZD-25-9]
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Soft rot caused by the pathogen Pectobacterium carotovorum subsp. carotovorum (Pcc) is a major factor limiting the development of the Amorphophallus spp. industry. This study compared two species of Amorphophallus and found that A. muelleri exhibited higher resistance to Pcc. The study also identified critical genes involved in resistance and provides a theoretical foundation for disease control through metabolic engineering.
Amorphophallus spp. are important industrial crops in Southwest China. Unfortunately, bacterial disease is one of the factors limiting the development of the Amorphophallus spp. industry. Soft rot can be caused by the pathogen Pectobacterium carotovorum subsp. carotovorum (Pcc) and leads to large-scale losses in Amorphophallus spp. quality and production. However, the mechanisms underlying the response of Amorphophallus spp. to Pcc remain largely unknown. This study compared two species of Amorphophallus, A. muelleri and A. konjac, to explore Pcc resistance by combined physiological and transcriptome analysis. On the basis of phenotype analysis, A. muelleri exhibited higher resistance to Pcc than A. konjac. Moreover, the transcriptomes of A. muelleri and A. konjac were assembled, and differentially expressed genes (DEGs) were annotated. Significant categories including the plant hormone transduction pathway, phenylpropanoid biosynthesis pathway and plant pathogen interaction pathway were identified. Among these pathways, small auxin upregulated RNA (SAUR), ethylene responsive transcription factor 1 (ERF1), 4-coumarate-CoA ligase (4CL), peroxidase (POD), WRKY transcription factor, Ca2+ sensor related and mitogen-activated protein kinase (MAPK) genes were upregulated in A. muelleri, which may be involved in the regulation of energy, oxidative stress and signal transduction upon exposure to Pcc infection. The transcription levels of four selected genes were further verified by quantitative real-time PCR. Overall, this study identifies several critical genes involved in resistance to soft rot disease in Amorphophallus spp. and provides a theoretical foundation for identifying potential targets for metabolic engineering for disease control.
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