Different subtype strains of Akkermansia muciniphila abundantly colonize in southern China

AimThis study investigates the colonization rate of Akkermansia muciniphila in the gastrointestinal tracts of people living in southern China and applies a modified method for the isolation and subtyping of A.muciniphila strains from faecal samples. Methods and ResultsFresh faecal samples were collected and bacterial DNA was extracted from these samples for real-time PCR analysis. Strains were separated using a culture-dependent sPCR-directed method and classified using an enterobacterial repetitive intergenic consensus (ERIC-PCR) DNA fingerprinting method. The colonization rate for the sample population from southern China was 5174%. We isolated 22 strains from human faeces. The results revealed that all strains were identifiable as A.muciniphila with 99-100% identity to the type-strain ATCC BAA-835. ERIC-PCR resulted in grouping of the DNA fingerprints showed that 12 distinct clusters were distinguished with a delineation level of 100%. ConclusionsSouthern China has a high rate of A.muciniphila colonization and over 12 different subtype strains reside in faecal samples. Significance and Impact of the StudyAkkermansia muciniphila has a beneficial role in human gastrointestinal tract. These studies provide a better understanding of A.muciniphila and details of its colonization in the human gastrointestinal tract.