Journal
PHYSICAL BIOLOGY
Volume 19, Issue 4, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1478-3975/ac70a1
Keywords
acoustic tweezers; neurite outgrowth; surface acoustic waves; cell viability; proliferation; B35 neuroblastoma cells
Categories
Funding
- cluster Nanosystems Initiative Munich
- Stiftung der Deutschen Wirtschaft
- Hanns-Seidel-Stiftung
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This research examines the impact of SSAW on the neurite outgrowth of B35 cells and explores the applicable range of SSAW parameters. The findings indicate that SSAW treatment significantly enhances the growth of neurites in preferential directions.
On the way towards neuronal stimulation and signalling, standing surface acoustic waves (SSAWs) have become a widely used technique to create well-defined networks of living cells in vitro during the past years. An overall challenge in this research area is to maintain cell viability in long-term treatments long enough to observe changes in cellular functions. To close this gap, we here investigate SSAW-directed neurite outgrowth of B35 (neuroblastoma) cells in microchannels on LiNbO3 chips, employing one-dimensional pulsed and continuous MHz-order SSAW signals at different intensities for up to 40 h. To increase the efficiency of future investigations, we explore the limits of applicable SSAW parameters by quantifying their viability and proliferation behaviour in this long-term setup. While cell viability is impaired for power levels above 15 dBm (32 mW), our investigations on SSAW-directed neurite outgrowth reveal a significant increase of neurites growing in preferential directions by up to 31.3% after 30 h of SSAW treatment.
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